Thermo Scientific PageRuler Prestained Protein Ladder, 10 to 180 kDa - Gel Electrophoresis Equipment and Supplies, Gel Electrophoresis Reagents and Buffers (2024)

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  1. Home
  2. Products
  3. Gel Electrophoresis Equipment and Supplies
  4. Gel Electrophoresis Reagents and Buffers
  5. Protein Molecular Weight Markers
  6. Thermo Scientific™PageRuler™ Prestained Protein Ladder, 10 to 180 kDa
  7. PI26616
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Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting.

Supplier: Thermo Scientific™ 26616

View more versions of this product

Catalog No. PI26616


Thermo Scientific PageRuler Prestained Protein Ladder, 10 to 180 kDa - Gel Electrophoresis Equipment and Supplies, Gel Electrophoresis Reagents and Buffers (6)

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  • Description
  • Specifications
  • Product Suggestions
  • Safety and Handling
  • SDS
  • Documents
  • Product Certifications
  • Promotions

Description

Description

Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Applications

  • Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
  • Monitoring protein transfer onto membranes after western blotting
  • Sizing of proteins on SDS-polyacrylamide gels and western blots

Thermo Scientific PageRuler Prestained Protein Ladder, 10 to 180 kDa - Gel Electrophoresis Equipment and Supplies, Gel Electrophoresis Reagents and Buffers (7)

Specifications

Specifications

Contents: two vials of 250 μL each

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C

10
Yes
3 colors: Blue, Orange, Green
180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa
2 x 250 μL
Western Blotting, SDS-PAGE
Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
Protein Ladder
10 to 180 kDa
Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
PageRuler™
Approved for shipment on Wet or Dry Ice

Product Suggestions

Product Suggestions

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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

SDS

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Thermo Scientific PageRuler Prestained Protein Ladder, 10 to 180 kDa - Gel Electrophoresis Equipment and Supplies, Gel Electrophoresis Reagents and Buffers (2024)

FAQs

What is prestained protein ladder? ›

The Prestained Protein Ladder is a three-color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 to 245 kDa.

What is the prestained protein standard? ›

The Color Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands (one orange and one green at 72 kDa and 26 kDa, respectively), that resolves into 11 sharp bands when electrophoresed.

How much protein ladder to load? ›

Transfer the desired amount of the Prestained Protein Ladder to a separate tube. For blotting: use 5 µl for mini-gels and 10 µl for full length gels. For visualizing during electrophoresis: use 10-15 µl for mini-gels and 20-30 µl for full length gels.

What is the difference between prestained and unstained? ›

Prestained standards allow direct visualization of the proteins' migration during electrophoresis and are useful to assess their subsequent transfer to membranes. Prestained standards can be used for size estimation, however unstained protein standards are recommended for the most accurate size determination.

What is prestained protein marker high range? ›

Color-coded Prestained Protein Marker, High Range (43-315 kDa) is a mixture of purified proteins, covalently coupled to blue, green, orange, or pink dyes, that resolves to 8 bands between 43 and 315 kDa when electrophoresed. The protein concentrations are carefully balanced for even intensity.

How much ladder to load in SDS-PAGE gel? ›

Load the following volumes of the ladder on an SDS-polyacrylamide gel: – 5 μL per well for mini gel, – 10 μL per well for large gel. Use the same volumes for Western blotting. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm.

Do you add a loading buffer to a ladder? ›

When the ladder is in the presence of water without loading dye, some of the DNA fragments can denature. This is why it is important to add the ladder to the diluted loading dye mixture. Alternatively, you can use TE buffer instead of water for any DNA ladder dilution to prevent DNA denaturation.

How much ladder do you need to load in DNA gel? ›

Load 5 μL of your DNA ladder per gel lane. (Your DNA ladder should preferably be in the first lane of your gel).

What is prestained protein ladder BenchMark? ›

Features of the BenchMark™ Pre-Stained Protein Ladder: Consists of 10 pre-stained protein bands in the range of 6–180 kDa (proteins are covalently coupled to a blue or pink dye) Includes a protein coupled to a pink dye (fourth band from the top) to ensure proper identification of each protein band.

What is Neb prestained protein standard? ›

The Color Prestained Protein Standard is designed for observing protein separation during SDS-PAGE, verification of western transfer efficiency on membranes, and for approximating the size of proteins (1-2). The Color Prestained Protein Standard may also be used in fluorescent imaging of SDS-PAGE.

What is the purpose of the Precision Plus protein Kaleidoscope Prestained Standard? ›

Recombinant prestained protein standards are carefully engineered for precise and accurate molecular weights. The staining process has been optimized to guarantee the same electrophoretic molecular weight with each lot. Traditional prestained proteins, on the other hand, are blended from naturally occurring proteins.

What is the significance of using a protein ladder in a SDS-PAGE? ›

Protein ladders or molecular weight markers are among the most commonly used reagents in biochemistry experiments. They provide molecular weight standards to estimate the size of proteins separated by gel electrophoresis like SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).

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